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Location： Home > Polyclonal Antibodies > Histone H2AX Rabbit Polyclonal Antibody

Histone H2AX Rabbit Polyclonal Antibody#53257

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Product Detail

Product NameHistone H2AX Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant protein of human Histone H2AX.

ConjugateUnconjugated

Other NamesH2A.X;H2A/X;H2AX;Histone H2AX;H2AFX;histone H2AX;gamma H2A.X;��H2AX

Accession NoSwiss Prot:P16104
Gene ID:3014

Uniprot P16104

Gene ID 3014

Calculated MW15kDa

Sdspage MW15kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:2000 - 1:5000
IHC 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Histone H2AX antibody.
Immunohistochemistry of paraffin-embedded rat testis using Histone H2AX antibody.
Immunohistochemistry of paraffin-embedded human colon carcinoma using Histone H2AX antibody.
Immunohistochemistry of paraffin-embedded mouse testis using Histone H2AX antibody.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

If you have published an article using product 53257, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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