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PDK4 Rabbit Polyclonal Antibody#53564

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Product Detail

Product NamePDK4 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human PDK4 (NP_002603.1).

ConjugateUnconjugated

Other NamesPDK4

Accession NoSwiss Prot:Q16654
Gene ID:5166

Uniprot Q16654

Gene ID 5166

Calculated MW46kDa

Sdspage MW46KDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using PDK4 antibody.
Immunohistochemistry of paraffin-embedded rat heart using PDK4 Rabbit pAb.
Immunohistochemistry of paraffin-embedded human placenta using PDK4 Rabbit pAb.
Immunohistochemistry of paraffin-embedded mouse heart using PDK4 Rabbit pAb.
Immunofluorescence analysis of C6 cells using PDK4 antibody.
Immunofluorescence analysis of L929 cells using PDK4 antibody.
Immunofluorescence analysis of U-2 OS cells using PDK4 antibody.
This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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