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DNMT1 Rabbit Polyclonal Antibody#53972

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Product NameDNMT1 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human DNMT1 (NP_001370.1).

ConjugateUnconjugated

Other NamesADCADN;AIM;CXXC9;DNMT;HSN1E;MCMT;m.HsaI;DNMT1

Accession NoSwiss Prot:P26358
Gene ID:1786

Uniprot P26358

Gene ID 1786

Calculated MW144kDa/183kDa/184kDa

Sdspage MW240kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:2000 - 1:5000
IHC 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Dnmt1 antibody.
Immunohistochemistry of paraffin-embedded rat spleen using DNMT1 antibody.
Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using DNMT1 antibody.
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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