Product Detail
Product NameCOL4A3 Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB,IF
Species ReactivityHuman,Mouse,Rat
Immunogen DescA synthetic peptide of human COL4A3 (NP_000082.2).
ConjugateUnconjugated
Other NamesCOL4A3
Accession NoSwiss Prot:Q01955
GeneID:1285
Uniprot
Q01955
Calculated MW135kDa/142kDa/158kDa/161kDa
Sdspage MW162kDa
FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of 293T cells, using COL4A3 at 1:1000 dilution.
Immunofluorescence analysis of L929 cells using COL4A3 at dilution of 1:100. Blue: DAPI for nuclear staining.
Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
If you have published an article using product 54194, please notify us so that we can cite your literature.
et al,Blocking ACSL6 Compromises Autophagy via FLI1‐Mediated Downregulation of COLs to Radiosensitize Lung Cancer. In Adv Sci (Weinh) on 2024 Oct by Wen Ding, Shijun Bao,et al..PMID:39206814
, (2024),
PMID:
39206814