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Location： Home > Polyclonal Antibodies > SEMA4A Rabbit Polyclonal Antibody

SEMA4A Rabbit Polyclonal Antibody#54393

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Product Detail

Product NameSEMA4A Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human SEMA4A (NP_071762.2).

ConjugateUnconjugated

Other NamesSEMA4A;CORD10;RP35;SEMAB;SEMB

Accession NoSwiss Prot:Q9H3S1
Gene ID:64218

Uniprot Q9H3S1

Gene ID 64218

Calculated MW69kDa/83kDa

Sdspage MW83kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of HT-29 cells, using SEMA4A Rabbit pAb.
Immunofluorescence analysis of C6 cells using SEMA4A antibody.
Immunofluorescence analysis of L929 cells using SEMA4A antibody.
This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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