Product Detail
Product NameLATS1 Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB,IHC
Species ReactivityHuman,Mouse,Rat
Immunogen DescRecombinant protein of human LATS1
ConjugateUnconjugated
Other NamesLATS1;WARTS;wts
Accession NoSwiss Prot:O95835
Gene ID:9113
Uniprot
O95835
Gene ID
9113
Calculated MW76kDa/126kDa
Sdspage MW130kDa
FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:2000 - 1:5000
IHC 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using LATS1 antibody.
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
If you have published an article using product 54491, please notify us so that we can cite your literature.
Yes
