Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Polyclonal Antibodies > Histone H2AX Rabbit Polyclonal Antibody

Histone H2AX Rabbit Polyclonal Antibody#54667

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(2) Other
Product Detail

Product NameHistone H2AX Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic peptide of human Histone H2AX.

ConjugateUnconjugated

Other NamesH2A.X;H2A/X;H2AX;Histone H2AX;H2AFX;histone H2AX;gamma H2A.X;γH2AX

Accession NoSwiss Prot:P16104
GeneID:3014

Uniprot P16104

Calculated MW15kDa

Sdspage MW15kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Immunofluorescence analysis of U-2 OS cells using Histone H2AX Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using Histone H2AX Polyclonal at dilution of 1:200. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Histone H2AX at 1:1000 dilution.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

If you have published an article using product 54667, please notify us so that we can cite your literature.

et al,2-Mercaptoethanol protects against DNA double-strand breaks after kidney ischemia and reperfusion injury through GPX4 upregulation. In Pharmacol Rep on 2022 Oct by Daeun Moon, Babu J Padanilam, et al..PMID: 35989399 , (2022),
PMID: 35989399
et al,AMBRA1 drives gastric cancer progression through regulation of tumor plasticity , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors