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Location： Home > Polyclonal Antibodies > Histone H3 Rabbit Polyclonal Antibody

Histone H3 Rabbit Polyclonal Antibody#55077

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Product NameHistone H3 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human HIST1H3D (NP_003520.1).

ConjugateUnconjugated

Other NamesHIST1H3D;H3/b;H3FB;histone H3.1;Histone H3;HIST1H3A

Accession NoUniprot:P68431
GeneID:8351

Uniprot P68431

Gene ID 8351

Calculated MW15kDa

Sdspage MW15kDa

FormulationPBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HIST1H3D antibody.
Immunohistochemistry of paraffin-embedded rat spleen using Histone H3 antibody.
Immunohistochemistry of paraffin-embedded human kidney cancer using Histone H3 antibody.
Immunohistochemistry of paraffin-embedded mouse cancer using Histone H3 antibody.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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