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KU70 Rabbit Polyclonal Antibody#55182

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Product Detail

Product NameKU70 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human KU70 (NP_001460.1).

ConjugateUnconjugated

Other NamesXRCC6;CTC75;CTCBF;G22P1;KU70;ML8;TLAA

Accession NoSwiss Prot:P12956
GeneID:2547

Uniprot P12956

Calculated MW65kDa/69kDa

Sdspage MW70kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:20 - 1:50
Immunohistochemistry of paraffin-embedded rat brain using KU70 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using KU70 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using KU70 at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using KU70 at 1:1000 dilution.
Immunofluorescence analysis of A549 cells using KU70 . Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U2OS cells using KU70 Polyclonal at dilution of 1:100. Blue: DAPI for nuclear staining.
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

If you have published an article using product 55182, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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