Product Detail
Product NamePTGS1 Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB,IHC,IF
Species ReactivityHuman,Mouse,Rat
Immunogen DescRecombinant fusion protein of human PTGS1 (NP_001258094.1).
ConjugateUnconjugated
Other NamesPTGS1;COX1;COX3;PCOX1;PES-1;PGG/HS;PGHS-1;PGHS1;PHS1;PTGHS
Accession NoSwiss Prot:P23219
GeneID:5742
Uniprot
P23219
Calculated MW56kDa/61kDa/64kDa/68kDa/71kDa/72kDa
Sdspage MW69kDa
FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Immunohistochemistry of paraffin-embedded human liver cancer using PTGS1 at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PTGS1 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 15s.
Immunofluorescence analysis of HeLa cells using PTGS1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants.
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