Product Detail
Product NameNAA60 Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB,IHC,IF
Species ReactivityHuman,Mouse,Rat
Immunogen DescRecombinant fusion protein of human NAA60 (NP_079121.1).
ConjugateUnconjugated
Other NamesNAA60;HAT4;NAT15;NatF;hNaa60
Accession NoSwiss Prot:Q9H7X0
GeneID:79903
Uniprot
Q9H7X0
Calculated MW19kDa/20kDa/27kDa/28kDa
Sdspage MW27kDa
FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Immunohistochemistry of paraffin-embedded human liver cancer using NAA60 at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using NAA60 at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric using NAA60 at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using NAA60 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele.
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