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CHEK1 Rabbit Polyclonal Antibody#55275

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Product Detail

Product NameCHEK1 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic peptide of human CHEK1

ConjugateUnconjugated

Other NamesCHEK1;CHK1

Accession NoSwiss Prot:O14757
GeneID:1111

Uniprot O14757

Calculated MW43kDa/50kDa/54kDa

Sdspage MW54kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using CHEK1 at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human kidney cancer using CHEK1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric using CHEK1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CHEK1 at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CHEK1 at dilution of 1:100. Blue: DAPI for nuclear staining.
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

If you have published an article using product 55275, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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