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SHC1 Rabbit Polyclonal Antibody#55300

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Product Detail

Product NameSHC1 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic peptide of human SHC1

ConjugateUnconjugated

Other NamesSHC;SHCA;SHC1

Accession NoSwiss Prot:P29353
GeneID:6464

Uniprot P29353

Calculated MW40kDa/46kDa/51kDa/62kDa

Sdspage MW46kDa, 52kDa, 66kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Immunohistochemistry of paraffin-embedded rat kidney using SHC1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using SHC1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SHC1 at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SHC1 at 1:1000 dilution.
Immunofluorescence analysis of U2OS cells using SHC1 at dilution of 1:100. Blue: DAPI for nuclear staining.
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

If you have published an article using product 55300, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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