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UBE2A Rabbit Polyclonal Antibody#55311

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Product Detail

Product NameUBE2A Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human UBE2A (NP_003327.2).

ConjugateUnconjugated

Other NamesUBE2A;HHR6A;MRXS30;MRXSN;RAD6A;UBC2

Accession NoSwiss Prot:P49459
GeneID:7319

Uniprot P49459

Calculated MW8kDa/13kDa/17kDa

Sdspage MW14kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using UBE2A at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse stomach using UBE2A (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using UBE2A (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using UBE2A (40x lens).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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