Product Detail
Product NameATG4D Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB,IF
Species ReactivityHuman,Mouse,Rat
Immunogen DescRecombinant fusion protein of human ATG4D (NP_116274.3).
ConjugateUnconjugated
Other NamesATG4D;APG4-D;APG4D;AUTL4
Accession NoSwiss Prot:Q86TL0
GeneID:84971
Uniprot
Q86TL0
Calculated MW16kDa/52kDa
Sdspage MW53kDa
FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ATG4D at 1:1000 dilution.
Immunofluorescence analysis of U2OS cells using ATG4D at dilution of 1:100. Blue: DAPI for nuclear staining.
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants.
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