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ACAT2 Rabbit Polyclonal Antibody#55351

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Product Detail

Product NameACAT2 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human ACAT2 (NP_005882.2).

ConjugateUnconjugated

Other NamesACAT2

Accession NoSwiss Prot:Q9BWD1
GeneID:39

Uniprot Q9BWD1

Calculated MW41kDa/44kDa

Sdspage MW41kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ACAT2 at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat ovary using ACAT2 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ACAT2 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using ACAT2 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ACAT2 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ACAT2 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using ACAT2 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using ACAT2 at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using ACAT2 at dilution of 1:100. Blue: DAPI for nuclear staining.
The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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