Product Detail
Product NameACO1 Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB
Species ReactivityHuman,Mouse,Rat
Immunogen DescRecombinant fusion protein of human IREB1 (NP_002188.1).
ConjugateUnconjugated
Other NamesACO1;ACONS;HEL60;IREB1;IREBP;IREBP1;IRP1
Accession NoUniprot:P21399
GeneID:48
Uniprot
P21399
Gene ID
48
Calculated MW98kDa
Sdspage MW98KDa
FormulationPBS with 0.02% sodium azide,50% glycerol,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:500 - 1:2000
Western blot analysis of extracts of various cell lines, using IREB1 antibody.
The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants
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