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CDH16 Rabbit Polyclonal Antibody#55359

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Product Detail

Product NameCDH16 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human CDH16 (NP_004053.1).

ConjugateUnconjugated

Other NamesCDH16

Accession NoSwiss Prot:O75309
GeneID:1014

Uniprot O75309

Calculated MW79kDa/85kDa/87kDa/89kDa

Sdspage MW110kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using CDH16 at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using CDH16 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDH16 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CDH16 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CDH16 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDH16 at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat kidney using CDH16 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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