Product Detail
Product NameADAM12 Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB,IHC,IF
Species ReactivityHuman,Mouse,Rat
Immunogen DescRecombinant fusion protein of human ADAM12 (NP_067673.2).
ConjugateUnconjugated
Other NamesADAM12;ADAM12-OT1;CAR10;MCMP;MCMPMltna;MLTN;MLTNA
Accession NoSwiss Prot:O43184
GeneID:8038
Uniprot
O43184
Calculated MW80kDa/99kDa
Sdspage MW100kDa
FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Immunofluorescence analysis of C6 cells using ADAM12 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using ADAM12 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ADAM12 at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ADAM12 at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat heart using ADAM12 at dilution of 1:100 (40x lens).
This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form.
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