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THOC1 Rabbit Polyclonal Antibody#55449

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Product Detail

Product NameTHOC1 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human THOC1 (NP_005122.2).

ConjugateUnconjugated

Other NamesTHOC1;HPR1;P84;P84N5

Accession NoSwiss Prot:Q96FV9
GeneID:9984

Uniprot Q96FV9

Calculated MW43kDa/75kDa

Sdspage MW92kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using THOC1 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using THOC1 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse kidney using THOC1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using THOC1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using THOC1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using THOC1 at dilution of 1:100 (40x lens).
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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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