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PTTG1 Rabbit Polyclonal Antibody#55474

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Product Detail

Product NamePTTG1 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human PTTG1 (NP_001269311.1).

ConjugateUnconjugated

Other NamesPTTG1;EAP1;HPTTG;PTTG;TUTR1;securin

Accession NoSwiss Prot:O95997
GeneID:9232

Uniprot O95997

Calculated MW22kDa

Sdspage MW24kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:100 - 1:200
Western blot analysis of extracts of various cell lines, using PTTG1 at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human lung cancer using PTTG1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using PTTG1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PTTG1 at dilution of 1:100 (40x lens).
The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene.

If you have published an article using product 55474, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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