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WNT5B Rabbit Polyclonal Antibody#55476

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Product Detail

Product NameWNT5B Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human WNT5B (NP_116031.1).

ConjugateUnconjugated

Other NamesWNT5B

Accession NoSwiss Prot:Q9H1J7
GeneID:81029

Uniprot Q9H1J7

Calculated MW40kDa

Sdspage MW50kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using WNT5B at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat lung using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat bone marrow using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis leydig using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using WNT5B at dilution of 1:100 (40x lens).
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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