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RNF5 Rabbit Polyclonal Antibody#55487

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Product Detail

Product NameRNF5 Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescRecombinant fusion protein of human RNF5 (NP_008844.1).

ConjugateUnconjugated

Other NamesRNF5;RING5;RMA1

Accession NoSwiss Prot:Q99942
GeneID:6048

Uniprot Q99942

Calculated MW19kDa

Sdspage MW21kDa

FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using RNF5 at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat liver using RNF5 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RNF5 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using RNF5 at dilution of 1:100 (40x lens).
The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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