Product Detail
Product NameMDC1 Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB,IHC,IF
Species ReactivityHuman
Immunogen DescRecombinant fusion protein of human MDC1 (NP_055456.2).
ConjugateUnconjugated
Other NamesMDC1;NFBD1
Accession NoSwiss Prot:Q14676
GeneID:9656
Uniprot
Q14676
Calculated MW116kDa/195kDa/197kDa/226kDa
Sdspage MW270kDa
FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Immunohistochemistry of paraffin-embedded human lung cancer using MDC1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using MDC1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using MDC1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using MDC1 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using MDC1 at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using MDC1 at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using MDC1 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 5s.
The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
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