Product Detail
Product NameFECH Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB,IHC,IF
Species ReactivityHuman,Mouse,Rat
Immunogen DescRecombinant fusion protein of human FECH (NP_000131.2).
ConjugateUnconjugated
Other NamesFECH;EPP;FCE
Accession NoSwiss Prot:P22830
GeneID:2235
Uniprot
P22830
Calculated MW47kDa/48kDa
Sdspage MW50kDa
FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Immunohistochemistry of paraffin-embedded human stomach using FECH at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using FECH at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using FECH at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using FECH at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using FECH at 1:1000 dilution.
The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.
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