Product Detail
Product NameMRPS16 Rabbit Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationAffinity purification
ApplicationsWB,IF
Species ReactivityHuman,Mouse,Rat
Immunogen DescRecombinant fusion protein of human MRPS16 (NP_057149.1).
ConjugateUnconjugated
Other NamesMRPS16;CGI-132;COXPD2;MRP-S16;RPMS16
Accession NoSwiss Prot:Q9Y3D3
GeneID:51021
Uniprot
Q9Y3D3
Calculated MW13kDa/15kDa
Sdspage MW15kDa
FormulationBuffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
StorageStore at -20˚C. Avoid freeze / thaw cycles.
Application Details
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using MRPS16 at 1:1000 dilution.
Confocal immunofluorescence analysis of U2OS cells using MRPS16 Polyclonal at dilution of 1:100. Blue: DAPI for nuclear staining.
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S16P family. The encoded protein is one of the most highly conserved ribosomal proteins between mammalian and yeast mitochondria. Three pseudogenes (located at 8q21.3, 20q13.32, 22q12-q13.1) for this gene have been described.
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