Yes
All lanes: GM130 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : 293 whole cell lysatesLane 3 : MCF7 whole cell lysatesLane 4 : K562 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 113 kDa
Observed band size: 130 kDa
Exposure time: 8 seconds
All lanes: GM130 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse lung lysates
Lane 2 : Mouse kidney lysatesLane 3 : Mouse skin lysatesLane 4 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 113 kDa
Observed band size: 130 kDa
Exposure time: 9 seconds
All lanes:GM130 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : GM130 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human cervix carcinoma tissue stained for GM130 using 56019 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence GM130 antibody (56019) ICC/IF staining of GM130 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56019 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
