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KD/KO Validation > Cathepsin L/V/K/H Rabbit mAb
Cathepsin L/V/K/H Rabbit mAb#56147
Yes
All lanes: Cathepsin L/V/K/H Rabbit mAb at 1/1k dilution
Lane 1 : HepG2 whole cell lysates Lane 2 : 3T3 whole cell lysates Lane 3 : PC12 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 37,38 kDa
Observed band size: 38 kDa
Exposure time: 7 seconds
All lanes:Cathepsin L/V/K/H Rabbit mAb at 1/1k dilution
Lane 1 : Cathepsin L/V/K/H knockdown Hela cell lysate
Lane 2 : Wild-type Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Cathepsin L/V/K/H using 56147 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human colon tissue stained for Cathepsin L/V/K/H using 56147 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Cathepsin L/V/K/H antibody (56147) ICC/IF staining of Cathepsin L/V/K/H in U-118 MG cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56147 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
