Yes
All lanes: Atg4B Rabbit mAb at 1/1k dilution
Lane 1 : Ramos whole cell lysates Lane 2 : 293 whole cell lysates Lane 3 : C6 whole cell lysates Lane 4 : Mouse brain lysates Lane 5 : Rat spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 5 seconds
All lanes:Atg4B Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : Atg4B knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence Atg4B antibody (56184) ICC/IF staining of Atg4B in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56184 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
