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Eph receptor B1 Rabbit mAb#56463

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Product Detail

Product NameEph receptor B1 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IP

Species ReactivityHuman Mouse Rat

SpecificityEph receptor B1 Antibody detects endogenous levels of total Eph receptor B1

Immunogen DescA synthesized peptide derived from human Eph receptor B1

ConjugateUnconjugated

Other NamesELK; NET; Hek6; EPHT2; EPHB1;

Accession NoUniprot:P54762

Uniprot P54762

Calculated MW120kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:2000
IP:1:50

All lanes: Eph receptor B1 Rabbit mAb at 1/1k dilution

Lane 1 : U-87 MG whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 110 kDa
Observed band size: 110 kDa

Exposure time: 6 seconds
All lanes:Eph receptor B1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : Eph receptor B1 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

If you have published an article using product 56463, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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