Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > KD/KO Validation > CLASP1 Rabbit mAb

CLASP1 Rabbit mAb#56478

Availability: Yes
Total Amount:$
Product Detail

Product NameCLASP1 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF FC

Species ReactivityMouse, Rat, Human

SpecificityCLASP1 Antibody detects endogenous levels of total CLASP1

Immunogen DescA synthesized peptide derived from human CLASP1

ConjugateUnconjugated

Other Namesclasp1; hOrbit1; MAST1;

Accession NoUniprot:Q7Z460

Uniprot Q7Z460

Calculated MW169kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:1000~1:5000
IHC:1:50~1:200
ICC/IF:1:50~1:200
FC:1:50

All lanes: CLASP1 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 169 kDa
Observed band size: 169 kDa

Exposure time: 7 seconds
All lanes:CLASP1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : CLASP1 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human gastric tissue stained for CLASP1 using 56478 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CLASP1 antibody (56478)

ICC/IF staining of CLASP1 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 56478 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 56478, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;