Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > KD/KO Validation > ABCE1 Rabbit mAb

ABCE1 Rabbit mAb#56617

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameABCE1 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF IP FC

Species ReactivityHuman Mouse Rat

SpecificityABCE1 Antibody detects endogenous levels of total ABCE1

Immunogen DescA synthesized peptide derived from human ABCE1

ConjugateUnconjugated

Other NamesABC38; ABCE1; HuHP68; OABP; RLI; RNASEL1; RNASELI; RNS4I;

Accession NoUniprot:P61221

Uniprot P61221

Calculated MW67kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB:1:1000~1:2000
IHC:1:100~1:200
ICC/IF:1:50~1:200
IP:1:50
FC:1:20~1:100
All lanes: ABCE1 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lane 4 : MCF7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure time: 6 seconds
All lanes: ABCE1 Rabbit mAb at 1/1k dilution

Lane 1 : Mouse liver lysates
Lane 2 : Mouse spleen lysates
Lane 3 : Mouse lung lysates
Lane 4 : Mouse kidney lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure time: 7 seconds
All lanes: ABCE1 Rabbit mAb at 1/1k dilution

Lane 1 : Rat heart lysates
Lane 2 : Rat liver lysates
Lane 3 : Rat spleen lysates
Lane 4 : Rat lung lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure time: 8 seconds
All lanes:ABCE1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : ABCE1 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for ABCE1 using 56617 at 1/100 dilution in immunohistochemical analysis.

 Nucleoside-triphosphatase (NTPase) involved in ribosome recycling by mediating ribosome disassembly.

If you have published an article using product 56617, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors