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CDKN2C Rabbit mAb#56630

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Product Detail

Product NameCDKN2C Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF IP

Species ReactivityHuman Mouse Rat

SpecificityCDKN2C Antibody detects endogenous levels of total CDKN2C

Immunogen DescA synthesized peptide derived from human CDKN2C

ConjugateUnconjugated

Other NamesCdkn2c; CDKN6; INK4C; p18; p18 inhibits CDK4; p18 INK4c; p18 INK6; p18-INK4c; p18-INK6;

Accession NoUniprot:P42773

Uniprot P42773

Calculated MW15kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:2000
IHC:1:50~1:200
ICC/IF:1:50~1:200
IP:1:40

All lanes: CDKN2C Rabbit mAb at 1/1k dilution

Lane 1 : MCF7 whole cell lysates
Lane 2 : SH-SY5Y whole cell lysates
Lane 3 : Mouse bone lysates
Lane 4 : Rat bone lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 18 kDa
Observed band size: 16 kDa

Exposure time: 8 seconds
All lanes:CDKN2C Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : CDKN2C knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for CDKN2C using 56630 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CDKN2C antibody (56630)

ICC/IF staining of CDKN2C in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 56630 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 56630, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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