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CC2D1A Rabbit mAb#56901

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Product Detail

Product NameCC2D1A Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB ICC/IF FC

Species ReactivityHuman Mouse Rat

SpecificityCC2D1A Antibody detects endogenous levels of total CC2D1A

Immunogen DescA synthesized peptide derived from human CC2D1A

ConjugateUnconjugated

Other NamesCoiled coil and C2 domain containing 1A; FREUD 1; Freud 1/Aki1; MRT3;

Accession NoUniprot:Q6P1N0

Uniprot Q6P1N0

Calculated MW130kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB:1:500~1:2000
ICC/IF:1:50~1:200
FC:1:50
All lanes: CC2D1A Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Mouse heart lysates
Lane 3 : Rat heart lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 104 kDa
Observed band size: 130 kDa

Exposure time: 5 seconds
All lanes :CC2D1A Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : CC2D1A knockdown HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CC2D1A using 56901 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CC2D1A antibody (56901)

ICC/IF staining of CC2D1A in MCF7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 56901 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 56901, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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