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KD/KO Validation > Histone H3 (acetyl K27) Rabbit mAb
Histone H3 (acetyl K27) Rabbit mAb#56909
Yes
All lanes: Histone H3 (acetyl K27) Rabbit mAb at 1/1k dilution
Lane 1 : HeLa cell treated with TSA cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 6 seconds
All lanes: Histone H3 (acetyl K27) Rabbit mAb at 1/1k dilution
Lane 1 : Mouse kidney lysates Lane 2 : Mouse brain lysates Lane 3 : Rat kidney lysates Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 8 seconds
All lanes:Histone H3 (acetyl K27) Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : Histone H3 (acetyl K27) knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human brain tissue stained for Histone H3 (acetyl K27) using 56909 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Histone H3 (acetyl K27) using 56909 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Histone H3 (acetyl K27) antibody (56909) ICC/IF staining of Histone H3 (acetyl K27) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56909 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
