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FACL4 Rabbit mAb#56910

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Product Detail

Product NameFACL4 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityFACL4 Antibody detects endogenous levels of total FACL4

Immunogen DescA synthesized peptide derived from FACL4

ConjugateUnconjugated

Other NamesACS4; Acsl4; acyl CoA synthetase 4; FACL4; Fatty acid Coenzyme A ligase; LACS4; Long chain 4; long chain fatty acid Coenzyme A ligase 4; MRX63; MRX68;

Accession NoUniprot:O60488

Uniprot O60488

Calculated MW79kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: FACL4 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : 293 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 79 kDa

Exposure time: 5 seconds
All lanes: FACL4 Rabbit mAb at 1/1k dilution

Lane 1 : Mouse liver lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat liver lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 79 kDa

Exposure time: 8 seconds
All lanes :FACL4 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : FACL4 knockdown A549 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for FACL4 using 56910 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for FACL4 using 56910 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat liver tissue stained for FACL4 using 56910 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence FACL4 antibody (56910)

ICC/IF staining of FACL4 in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 56910 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 56910, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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