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AP2S1 Rabbit mAb#56927

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Product Detail

Product NameAP2S1 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC IP

Species ReactivityHuman Mouse Rat

SpecificityAP2S1 Antibody detects endogenous levels of total AP2S1

Immunogen DescA synthesized peptide derived from AP2S1

ConjugateUnconjugated

Other NamesAP17; Ap2s1; CLAPS2; Sigma adaptin 3b; Sigma2 adaptin;

Accession NoUniprot:P53680

Uniprot P53680

Calculated MW17kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB:1:500~1:2000
IHC:1:50~1:200
IP:1:50
All lanes: AP2S1 Rabbit mAb at 1/1k dilution

Lane 1 : 293 whole cell lysates
Lane 2 : Mouse brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 17 kDa
Observed band size: 17 kDa

Exposure time: 6 seconds
All lanes: AP2S1 Rabbit mAb at 1/1k dilution

Lane 1 : 3T3 whole cell lysates
Lane 2 : PC12 whole cell lysates
Lane 2 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 17 kDa
Observed band size: 17 kDa

Exposure time: 8 seconds
All lanes:AP2S1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :AP2S1 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for AP2S1 using 56927 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human thyroid cancer tissue stained for AP2S1 using 56927 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 56927, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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