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ACAT2 Rabbit mAb#56976

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Product Detail

Product NameACAT2 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificityACAT2 Antibody detects endogenous levels of total ACAT2

Immunogen DescA synthesized peptide derived from human ACAT2

ConjugateUnconjugated

Other NamesAcat2; ACTL;

Accession NoUniprot:Q9BWD1

Uniprot Q9BWD1

Calculated MW38kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:2000
IHC:1:50~1:200

All lanes: ACAT2 Rabbit mAb at 1/1k dilution

Lane 1 : K562 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 41 kDa
Observed band size: 38 kDa

Exposure time: 5 seconds
All lanes: ACAT2 Rabbit mAb at 1/1k dilution

Lane 1 : Rat liver lysates
Lane 2 : Rat kidney lysates
Lane 3 : Mouse spleen lysates
Lane 4 : Mouse lung lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 41 kDa
Observed band size: 38 kDa

Exposure time: 6 seconds
All lanes :ACAT2 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : ACAT2 knockdown A549 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human prostate tissue stained for ACAT2 using 56976 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 56976, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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