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HRPT2 / CDC73 Rabbit mAb#57154

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Product Detail

Product NameHRPT2 / CDC73 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Mouse Rat

SpecificityHRPT2 / CDC73 Antibody detects endogenous levels of total HRPT2 / CDC73

Immunogen Desc A synthesized peptide derived from human HRPT2 / CDC73

ConjugateUnconjugated

Other NamesCdc73; FIHP; HPTJT; HRPT1; HRPT2; Hyperparathyroidism 2; HYX;

Accession NoQ6P1J9

Calculated MW61kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details

  WB 1:500~1:2000

All lanes: HRPT2 / CDC73 Rabbit mAb at 1/1k dilution

Lane 1 : 293 whole cell lysates
Lane 2 : MCF-7 whole cell lysates
Lane 3 : K562 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa

Exposure time: 5 seconds
All lanes: HRPT2 / CDC73 Rabbit mAb at 1/1k dilution

Lane 1 : Mouse lung lysates
Lane 2 : Mouse kidney lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lane 5 : Rat heart lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa

Exposure time: 5 seconds
All lanes:HRPT2 / CDC73 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : HRPT2 / CDC73 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for HRPT2 / CDC73 using 57154 at 1/100 dilution in immunohistochemical analysis.

  Tumor suppressor probably involved in transcriptional and post-transcriptional control pathways. May be involved in cell cycle progression through the regulation of cyclin D1/PRAD1 expression.

If you have published an article using product 57154, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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