Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > Recombinant Tau(Phospho-Thr217) Rabbit mAb(G122)

Recombinant Tau(Phospho-Thr217) Rabbit mAb(G122)#58004

Datasheet PDF
Availability： yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameRecombinant Tau(Phospho-Thr217) Rabbit mAb(G122)

Clone No.G122

Host SpeciesRabbit

ClonalityMonoclonal

PurificationAffinity purification

ApplicationsELISA,WB,IHC,IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous levels of Tau only when phosphorylated at threonione 217.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 217(L-P-T(p)-P-P) derived from Human Tau.

Target NameTau

ConjugateUnconjugated

Other NamesMAPT; MTBT1; PHF-tau;

Accession NoSwiss-Prot#: P10636;
NCBI Gene#: 4137;
NCBI Protein#: NP_058519.3.

Uniprot P10636

Sdspage MW50-80kd

Concentration1.0 mg/ml

FormulationLiquid in Tris-Gly containing 50% glycerol and 0.02% sodium azide.

StorageStore at -20°C. Avoid freeze/thaw cycles.

Application Details
Elisa:1:10000
WB: 1:500~1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Western blot analysis of extracts from 293 cells, transfected with Tau and treated with calf intestinal phosphatase (CIP) using Tau(Phospho-Thr217) Rabbit mAb.
Formalin-fixed, paraffin-embedded Mouse brain tissue stained for Tau(Phospho-Thr217) using 58004 at 1/50 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Tau(Phospho-Thr217) antibody (58004)

ICC/IF staining of Tau(Phospho-Thr217) in mouse brain tissue. mouse brain was fixed with 4% Paraformaldehyde permeabilized for 20 minutes.
Samples were incubated with 58004 at a working dilution of 1/50. The secondary antibody was Alexa Fluor 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence Tau(Phospho-Thr217) antibody (58004)

ICC/IF staining of Tau(Phospho-Thr217) in mouse brain tissue. mouse brain was fixed with 4% Paraformaldehyde permeabilized for 20 minutes.
Samples were incubated with 58004 at a working dilution of 1/50. The secondary antibody was Alexa Fluor 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

 Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. 

Goedert M., Proc. Natl. Acad. Sci. U.S.A. 85:4051-4055(1988).
Goedert M., EMBO J. 8:393-399(1989).
Lee G., Neuron 2:1615-1624(1989).

If you have published an article using product 58004, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors