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Insig1/2(Phospho-Ser207/151) Antibody#58005

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Product Detail

Product NameInsig1/2(Phospho-Ser207/151) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

ApplicationsWB IHC

Species ReactivityHu

SpecificityInsig1/2(Phospho-Ser207/151) antibody detects endogenous levels of Insig1/2 only when phosphorylated at serine207/151 .

Immunogen DescThe antiserum was produced against synthesized phosphopeptide derived from Human Insig1/2 around the phosphorylation site of serine 207/151

Accession No:NCBI Gene ID: 3638 (Insig1); NCBI Gene ID: 51141 (Insig2)

Uniprot :NCBI Gene ID: 3638 (Insig1); NCBI Gene ID: 51141 (Insig2)

Calculated MWPredicted MW: Insig1 pS207: 30~40 KD Insig2 pS151: ~25 KD

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

StorageStore at -20℃/1 year

Application Details

WB dilution: 1:500~1:1000

IHC dilution: 1:50-100

Huh7 cells expressing Flag-Insig1 (left panel) or Flag-Insig2 (right panel) were treated with or without IGF1 (100 ng/ml) for 1 h. Immunoprecipitation and immunoblotting analyses were performed with the indicated antibodies in the presence or absence of a blocking peptide for Insig1 pS207 and Insig2 pS151.
IHC analyses of human HCC samples were performed with the indicated antibodies in the presence or absence of a blocking peptide for Insig1 pS207 and Insig2 pS151.

If you have published an article using product 58005, please notify us so that we can cite your literature.

et al,Porcine reproductive and respiratory syndrome virus activates lipid synthesis through a ROS-dependent AKT/PCK1/INSIG/SREBPs axis. In Int J Biol Macromol on 2024 Dec by Ying-Xian Ma, Ya-Qi Han,et al..PMID:39433189 , (2024),
PMID: 39433189
et al,HSPA8 dampens SCAP/INSIG split and SREBP activation by reducing PKR-mediated INSIG phosphorylation. , (2025),
PMID: 39977267

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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