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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Ku70 Rabbit mAb

Ku70 Rabbit mAb#59402

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Product Detail

Product NameKu70 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman

SpecificityKu70 Antibody detects endogenous levels of total Ku70

Immunogen DescA synthesized peptide derived from human Ku70

Other NamesCTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku 70; Ku autoantigen 70kDa; Ku autoantigen, 70kDa; Ku p70; Ku70; Kup70; TLAA; XRCC6;

Accession NoUniprot:P12956

Uniprot P12956

Calculated MWPredicted band size: 70 kDa

Sdspage MWObserved band size: 70 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Ku70 Rabbit mAb at 1/1k dilution

Lane 1 : JK whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lane 3 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 3 seconds
All lanes:Ku70 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :Ku70 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ku70 using 59402 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Ku70 antibody (59402)

ICC/IF staining of Ku70 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59402 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together.

If you have published an article using product 59402, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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