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TDP43 Rabbit mAb#59416

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Product Detail

Product NameTDP43 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat Zebrafish

SpecificityTDP43 Antibody detects endogenous levels of total TDP43

Immunogen DescA synthesized peptide derived from human TDP43

Other NamesALS10; TAR DNA binding protein 43; TARDBP; TDP43;

Accession NoUniprot:Q13148

Uniprot Q13148

Calculated MWPredicted band size: 45 kDa

Sdspage MWObserved band size: 45 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: TDP43 Rabbit mAb at 1/1k dilution

Lane 1 : K562 whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 45 kDa

Exposure time: 7 seconds
All lanes:TDP43 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :TDP43 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human glioma tissue stained for TDP43 using 59416 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence TDP43 antibody (59416)

ICC/IF staining of TDP43 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59416 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
TDP43 (TAR DNA-binding protein 43) is involved in transcriptional regulation and exon splicing. While normal TDP43 is a nuclear protein, pathological TDP43 is a component of insoluble aggregates in patients with frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS).

If you have published an article using product 59416, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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