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Location： Home > Recombinant Rabbit Monoclonal Antibodies > S100 Rabbit mAb

S100 Rabbit mAb#59432

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Product Detail

Product NameS100 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC IP FC

Species ReactivityHuman Mouse

SpecificityS100 Antibody detects endogenous levels of total S100

Immunogen DescA synthesized peptide derived from human S100

Other NamesBpb; NEF; Protein S100-A1; S100 beta; S100 calcium binding protein A1; S100A; S100B; 100beta;

Accession NoUniprot:P23297

Uniprot P23297

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB 1:500~1:1000 IHC 1:100~1:500 IP 1:50 FC 1:50
Western blot analysis of S100 expression in (1) Human skeletal muscle lysate; (2) RAW 264.7 cell lysate.
Immunohistochemical analysis of paraffin-embedded human kidney, using S100 Antibody.
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

If you have published an article using product 59432, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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