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PARP Rabbit mAb#59433

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Product Detail

Product NamePARP Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityPARP Antibody detects endogenous levels of total PARP

Immunogen DescA synthesized peptide derived from human PARP

Other NamesPPOL; ADPRT; ADPRT1; pADPRT-1; PARP; PARP-1;

Accession NoUniprot:P09874

Uniprot P09874

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB 1:500~1:1000 IHC 1:100~1:500 ICC/IF 1:50~1:200
Western blot analysis of PARP expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with staurosporine.
Immunohistochemical analysis of paraffin-embedded mouse testis, using PARP Antibody.
Immunofluorescent analysis of Hela cells, using PARP Antibody .
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.

If you have published an article using product 59433, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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