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Drosha Rabbit mAb#59448

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Product Detail

Product NameDrosha Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman

SpecificityDrosha Antibody detects endogenous levels of total Drosha

Immunogen DescA synthesized peptide derived from human Drosha

Other NamesRibonuclease 3 (EC:3.1.26.3); Protein Drosha; Ribonuclease III; RNase III; p241; DROSHA; RN3; RNASE3L; RNASEN;

Accession NoUniprot:Q9NRR4

Uniprot Q9NRR4

Calculated MWPredicted band size: 159 kDa

Sdspage MWObserved band size: 159 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Drosha Rabbit mAb at 1/1k dilution

Lane 1 : 293 whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 159 kDa
Observed band size: 159 kDa

Exposure time: 11 seconds
All lanes:Drosha Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :Drosha Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Drosha using 59448 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Drosha antibody (59448)

ICC/IF staining of Drosha in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59448 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing. This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs. Drosha exists in a multiprotein complex called Microprocessor along with other components such as DGCR8. Drosha, along with DGCR8, is necessary for miRNA biogenesis.

If you have published an article using product 59448, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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