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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Sortilin Rabbit mAb

Sortilin Rabbit mAb#59471

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Product Detail

Product NameSortilin Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificitySortilin Antibody detects endogenous levels of total Sortilin

Immunogen DescA synthesized peptide derived from human Sortilin

Other Names100 kDa NT receptor; Glycoprotein 95; Gp95; LDLCQ6; Neurotensin receptor 3; NT3; NTR3; SORT 1; Sortilin 1;

Accession NoUniprot:Q99523

Uniprot Q99523

Calculated MWPredicted band size: 92 kDa

Sdspage MWObserved band size: 110 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: Sortilin Rabbit mAb at 1/1k dilution

Lane 1 : SW480 whole cell lysates
Lane 2 : Hela whole cell lysates
Lane 3 : JK whole cell lysates
Lane 4 : C6 whole cell lysates
Lane 5 : Mouse kidney lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 92 kDa
Observed band size: 110 kDa

Exposure time: 7 seconds
All lanes:Sortilin Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 :Sortilin Rabbit mAb knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human brain tissue stained for Sortilin using 59471 at 1/100 dilution in immunohistochemical analysis.
Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR).

If you have published an article using product 59471, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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