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Location： Home > Recombinant Rabbit Monoclonal Antibodies > GC1q R Rabbit mAb

GC1q R Rabbit mAb#59527

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Product Detail

Product NameGC1q R Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityGC1q R Antibody detects endogenous levels of total GC1q R

Immunogen DescA synthesized peptide derived from human GC1q R

Other NamesASF/SF2 associated protein p32; C1q globular domain binding protein; C1qBP; GC1Q R; GC1QBP; GC1QR; p32 splicing factor; SF2p32;

Accession NoUniprot:Q07021

Uniprot Q07021

Calculated MWPredicted band size: 31 kDa

Sdspage MWObserved band size: 31 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: GC1q R Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lane 3 : PC12 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 31 kDa

Exposure time: 7 seconds
All lanes:GC1q R Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 :GC1q R Rabbit mAb knockdown HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for GC1q R using 59527 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence GC1q R antibody (59527)

ICC/IF staining of GC1q R in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59527 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Binds to the globular heads" of C1Q thus inhibiting C1 activation.,,Q07021,Null,http://www.uniprot.org/uniprot/Q07021,Null324195,324195,59528,Null,CXCR3 Rabbit mAb,Rabbit,Monoclonal,Null,Rabbit IgG,Affinity-chromatography,WB ICC/IF IP,WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50,Human,CXCR3 Antibody detects endogenous levels of total CXCR3,,,,,C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; CKR-L2; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor; CD183; GPR9; CXCR3;,Uniprot:P49682,,Null,Null,41kDa,Null,Null,Null,Null,Null,Null,A synthesized peptide derived from human CXCR3,Null,Null,Null,Null,Null,Null,Null,Null,Null,Null,Null,"Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

If you have published an article using product 59527, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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