Product Detail
Product NameAtg7(Apg7) Rabbit mAb
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
PurificationAffinity-chromatography
ApplicationsWB ICC/IF
Species ReactivityHuman Mouse Rat
SpecificityAtg7(Apg7) Antibody detects endogenous levels of total Atg7(Apg7)
Immunogen DescA synthesized peptide derived from human Atg7(Apg7)
Other NameshAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L;
Accession NoUniprot:O95352
Uniprot
O95352
FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.
Application Details
WB 1:1000~1:5000 ICC/IF 1:50~1:200
Western blot analysis of Atg7(Apg7) expression in (1) HepG2 cell lysate; (2) Mouse spleen lysate; (3) Rat kidney lysate.
All lanes : ATG7 Rabbit mAb at 1/1k dilution Lane 1 : Wild-type HAP1 cell lysate Lane 2 : ATG7 knockdown HAP1 cell lysate Lysates/proteins at 20 µg per lane.
Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.
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