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RBPJK Rabbit mAb#59717

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Product Detail

Product NameRBPJK Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityRBPJK Antibody detects endogenous levels of total RBPJK

Immunogen DescA synthesized peptide derived from human RBPJK

Other NamesSUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1;

Accession NoUniprot:Q06330

Uniprot Q06330

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB 1:500~1:2000 ICC/IF 1:50~1:200
Western blot analysis of RBPJK expression in MCF7 cell lysate.
All lanes: RBPJK Rabbit mAb at 1/1k dilution
Lane 1 : JK whole cell lysates
Lane 2 : MCF-7 whole cell lysates
Lane 3 : C6 whole cell lysates
Lane 4 : Raw264.7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa
Exposure time: 4 seconds
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins.

If you have published an article using product 59717, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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